Bioavailability of CBD Greatly Increased with ANANDA Scientific’s Nano-Enhanced CBD

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Bioavailability of CBD Greatly Increased with ANANDA Scientific’s Nano-Enhanced CBD

Cannabidiol (CBD) from industrial hemp is a multi-functional molecule, and the bioavailability of CBD is greatly increased because of ANANDA.
Scientific studies indicated that it may be a more powerful antioxidant than either Vitamin C or E, and CBD offers the prospect of successfully fighting chronic inflammation and protecting brain cells from reactive oxygen species (1-2).

CBD’s beneficial potential is discussed in numerous published papers. Further, it has promise in stabilizing and even reducing blood sugar levels; as a pain killer; for reducing the risk of artery blockage; in suppressing muscle spasms, seizures, and convulsions; for fighting varied cancers; and more (3-8).

Such promise is accompanied by a major limitation to its usefulness — low bioavailability. Because of this, any beneficial effects from CBD become patchy or erratic due to problems in getting CBD into the body in adequate amounts (9-14).

For a supplement taken by mouth, bioavailability means the proportion of a dose that enters the bloodstream from the small intestine (15-17). Therefore, once in the blood, the supplement can find its way to the target organ or body system, where it then goes to work in supporting health and wellness.

On average, only 5-6% of almost any CBD preparation gets into the bloodstream. As a result, the rest is wasted. Such poor oral bioavailability guarantees variable or unpredictable effects, along with increased costs from having to take larger doses to compensate.

Appropriate formulation strategies that assist in getting into the bloodstream are thus mandatory for CBD to attain its health-giving potential, as well as in a cost-efficient or economical fashion.

ANANDA Scientific’s research & development has yielded a patented CBD technology using GRAS ingredients that resolve CBD’s bioavailability problem. This patented technology is the first of its kind. “GRAS” means that a substance is Generally Recognized As Safe by the US Food and Drug Administration to be used in foods and beverages (18).

Comparison of bioavailability of CBD

Figure 1. ANANDA Scientific’s patented, proprietary technology (nextCBD) involves highly-ordered constructs made from GRAS compounds into which CBD is affixed. Therefore, this technology makes nextCBD very bioavailable when taken by mouth.

Purpose.

This study compares the bioavailability of CBD and ANANDA Scientific’s enhanced CBD in laboratory rats. The bioavailability of substances taken by mouth are comparable between rats and humans (19-28).

Methods.

This demonstration looks at the plasma contents of cannabidiol (CBD) after a single oral dose administered by gavage (through a tube leading down the throat to the stomach; 29) of regular CBD and ANANDA Scientific’s enhanced CBD over a 24-hour period.

In this example, female Sprague-Dawley rats (240-265 gm body weight) were used. The study design and animal usage were reviewed and approved by an Institutional Animal Care and Use Committee (IACUC) for compliance with regulations prior to study initiation. Animal welfare for this study with the U.S. Department of Agriculture’s (USDA) Animal Welfare Act (9 CFR Parts 1, 2, and 3) and the Guide for the Care and Use of Laboratory Animals (30).

A 50-mg CBD/kg body weight model was examined in animals given ANANDA Scientific’s nano-enhanced pure CBD and a control group for which powdered pure CBD in the same amount was fed. Ten animals were in each group.

Blood samples were taken immediately prior to gavage as well as 0.5, 1.0, 2.0, 4.0, 8.0, 12.0 and 24.0 hours after dosing. Venous blood was collected in an EDTA blood collection tube. Next, plasma was separated from red blood cells by centrifugation at 400 g for 15 min., transferred to a fresh microcentrifuge tube, and stored at −80°C.

CBD was quantified using validated high-performance liquid chromatography with tandem mass spectroscopy (LC-MS-MS) in multiple reaction monitoring (MRM) mode.

Findings.

The results verify that ANANDA’s enhanced methods greatly improves bioavailability of CBD. It was significantly more bioavailable than regular CBD at 0.5 and 2 hours.

As a result, far lower dosing is needed for enhanced CBD versus regular CBD. The results also intimate that products containing the regular, non-enhanced CBD found in most products may suffer from low bioavailability and a consequent ineffectiveness.

Study of the bioavailibility of CBD over time

Contact us today to get started with all your CBD needs.

References

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2. Couch, D.G., H. Maudslay, B. Doleman, J.N. Lund, and S.E. O’Sullivan. 2018. The use of cannabinoids in colitis: a systematic review and meta-analysis. Inflammatory Bowel Disease 24(4):680-697.

3. Campos, A.C., M.V. Fogaça, A.B. Sonego, and F.S. Guimarães. 2016. Cannabidiol, neuroprotection and neuropsychiatric disorders. Pharmacological Research 112:119-127.


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5. McAllister, S.D., L. Soroceanu, and P.Y. Desprez. 2015. The antitumor activity of plant-derived non-psychoactive cannabinoids. Journal of Neuroimmune Pharmacology 10(2):255-267.

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7. Robson, P.J. . 2014. Therapeutic potential of cannabinoid medicines. Drug Testing and Analysis 6(1-2):24-30.

8. Russo, E.B. 2008. Cannabinoids in the management of difficult to treat pain. Therapeutics and Clinical Risk Management 4(1):245-259.

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13. Ohisson, A., J.E. Lindgren, S. Andersson, S. Agurell, H. Gillespie, L.E. Hollister. 1986. Single-dose kinetics of deuterium-labeled cannabidiol in man after smoking and intravenous administration. Biomed Environ Mass Spectrometry 13:77–83.

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15. Bhattaram, V.A., U. Graefe, C. Kohlert, and H. Derendorf. 2002. Pharmacokinetics and bioavailability of herbal medicinal products. Phytomedicine 9 (Suppl 3):1-33.

16. El-Kattan, A.F. 2017. Oral Bioavailability Assessment: Basics and Strategies for Drug Discovery and Development (Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods). First Edition. Wiley, New York, 448 p.

17. Hu, M., and X. Li. 2011. Oral Bioavailability: Basic Principles, Advanced Concepts, and Applications. First Edition. Wiley, New York, 568 p. 18. GRAS Substances (SCOGS) Database. U.S. Food and Drug Administration. https://www.fda.gov/Food/IngredientsPackagingLabeling/GRAS/SCOGS

19. Akonur, A.I., C.J. Holmes, and J.k. Leypoldt. 2014. Predicting the peritoneal absorption of icodextrin in rats and humans including the effect of β-amylase activity in dialysate. Peritoneal Dialysis International 35(3)288-296.

20. Fagerholm, U., M. Johansson, and H. Lennernäs. 1996. Comparison Between Permeability Coefficients in Rat and Human Jejunum. Pharmaceutical Research 13(9):1336-1342.

21. Lawless E., B.T. Griffin B, A. O’Mahony A, and C.M. O’Driscoll. 2015. Exploring the impact of drug properties on the extent of intestinal lymphatic transport – in vitro and in vivo studies. Pharmaceutical Research 32(50:1817-1829.

22. Nagahara, N., Y. Akiyama, K. Higaki, and T. Kimura. 2006. Animal models for predicting potency of oral sustained-release adhesive microspheres in humans. International Journal of Pharmacy 331(1):46-53.

23. Pang, K.S. 2003. Modeling of intestinal drug absorption: roles of transporters and metabolic enzymes. Drug Metabolism and Disposition 31(12):1509-1517.

24. Salphati, L., K. Childers, L. Pan, K. Tsutsui, and L. Takahashi. 2001. Evaluation of a single-pass intestinal-perfusion method in rat for the prediction of absorption in man. Journal of Pharmacy and Pharmacology 53(7):1007-1013.

25. Stewart, B,H., O.H. Chan, R.H. Lu, E.L. Reyner, H.L. Schmid, H.W. Hamilton, B.A. Steinbaugh, and M.D. Taylor. 1995. Comparison of intestinal permeabilities determined in multiple in vitro and in situ models: relationship to absorption in humans. Pharmaceutical Research 12(5):693-699.

26. Zenghui Teng , Z., C. Yuan , F. Zhang, M. Huan, W. Cao, K. Li, J. Yang, D. Cao, S. Zhou, and Q. Mei. 2012. Intestinal absorption and first-pass metabolism of polyphenol compounds in rat and their transport dynamics in Caco-2 cells. PLoS One 7(1):e29647.

27. Zakeri-Milania,P., H. Valizadeha, H. Tajerzadehc, Y. Azarmia, Z. Islambolchilara, S. Barzegara, and M. Barzegar-Jalalia. 2007. Predicting human intestinal permeability using single-pass intestinal perfusion in rats. International Journal of Pharmacy and Pharmaceutical Sciences 10(3):368-379.

28. Zhang, D., and L._X. Gang. 2012. Preclinical experimental models of drug metabolism and disposition in drug discovery and development. Acta Pharmaceutica Sinica B 2(6):549-561.

29. Andrews, K., and S. McErla. 2012. Oral dosing (gavage) in adult mice and rats. University of British Columbia Animal Care Guidelines, Standard Operating Procedure (SOP) ACC-2012-Tech09.

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Challenges and Solutions to Flavoring CBD Products

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Challenges and Solutions to Flavoring CBD Products

During the last few years, there’s been challenges in getting CBD products into marketing, and more importantly, good flavoring of CBD. In 2018, most CBD products were tinctures, vapes, softgels or capsules, gummies, dog chews, equine pellets, or in the skincare and cosmetics form.

Back in September 2019, Senate Majority Leader Mitch McConnell (R-KY) moved to insert language into a congressional spending report that calls on the Food and Drug Administration (FDA) to clear a path for the lawful marketing of hemp-derived CBD products.

In draft language shared by the U.S. Hemp Roundtable on Tuesday, the senator is asking FDA to “issue a policy of enforcement discretion with regard to certain products containing CBD” within 120 days. Industry stakeholders say this will clarify rules so that banks are more willing to service CBD companies.

Oil versus powder flavoring CBD

Hydrophobic vs. Oil-based Products

Starting out, all CBD products were hydrophobic/oil-based products. CBD was initially available in two main forms- curde or purified oil extract, or oil soluble powder. These oil-based products were the easiest to make, and required the least amount of effort.  All of the previous applications for CBS were the path of least resistance. With the passage of the 2018 Farm Bill, there was a flood of companies looking to be the first to market. The target audience for CBD products were all true CBD believers. Taste was less important than the medicinal properties of it.

As we move into the next generation of CBD products, we’re seeing more variety in the forms that CBD is offered in such as isolate, powdered, crystal, oil, and more. This variety in forms allows greater control over potency and purity. We’re moving from purely “Medicinal” products to more “Lifestyle” product categories. Some new lifestyle products we’re seeing are RTD Beverages, Shots, Infusions, Sleep Aides, Teas, and Lotions. We’re also seeing food products like mints, candies, gum, and chews.

Flavoring CBD graphEarly adopters with flavoring CBDWater Soluble CBD

Unlike the first generation of oil-based products, new water soluble CBD helps reduce stability problems and increases bioavailability. More refined CBD means less variation in consistency. A lower potency with water soluble products makes it easier to flavor these products.

Growing the category from just the “True Believers” and “Innovators” to begin to serve the “Early Adopters” requires a shift in product development. However, there’s a few hurdles in order to move past that initial 2.5% of the market. Kristen Nichols, from MJBiz Magazine, said, “There’s more to crafting a winning CBD beverage than figuring out how to get the cannabinoid into liquid, though. The bigger challenge is making a beverage people want to drink. Consumers want a tasty beverage that competes against fruit juices or heavily sweetened energy drinks, not something that tastes like medicine.”

Guide to terpines and flavoring CBD

Terpenes

This is where terpenes enter the picture. Terpenes are aromatic oils found in many plants. These  include cannabis varieties that can have distinctive flavors like citrus, berry, mint, piney, nutty, grassy and bitter. Over 100 different terpenes have been identified in the cannabis plant, and every strain tends toward a unique terpene type and composition. There are many factors that influence the taste profile of cannabinoids, including climate, weather, age and maturation, fertilizers, and soil type. Luckily, terpenes can be removed from CBD, but it’s easier to work with them than against them. For example, caryophyllene has a spicy, woody, pepper aroma. Some sample flavors that work with peppery terpenes are peppermint candy, mango chili pepper, cinnamon clove, and hot tamale.

When working with flavors and CBD, formulation will be specific to the strain, source, and delivery method. What you are tasting in CBD products… isn’t pure CBD. It’s the associated terpenes, compounds, chemicals and carriers of that particular ingredient source. As production and processing of CBD improves, the flavor profiles of the CBD of tomorrow won’t be the same as the CBD of today.

Flavoring CBD is important to create a good product

If you have unwanted taste in your CBD, an effective way to block unwanted taste attribute is to confuse the tongue with both different and like sensations. Bitter blockers can be sweet, sour, salt, bitter, or umami. Stevia and monk fruit or malic acic can also mask the taste. It’s important to consider the concentration of your CBD. Flavoring a 10% CBD solution is 90% easier than flavoring an 80% CBD solution. In order to flavor a solution, you must consider dilutions, carriers, delivery method, and emulsions. You must also consider water soluble ingredients that you choose to add to your CBD.

In Conclusion

There’s lots of aspects that go into making a good CBD product. With so many changes happening in the industry, Sensapure can be a great resource to help perfect your product. Send your approved CBD ingredient to our flavor lab to use as the source of your CBD product. Its use in a base product can make a significant difference in determining the best flavor solution. Give us a call to schedule a consult today.

Food Industry Regulation: In-Depth with Labels

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Food Industry Regulation: In-Depth with Labels

Food is all about flavor. It’s an important characteristic of the food we eat every day. You know how fresh foods taste when you make them at home? Fresh bread right from the oven, fresh squeezed orange juice, warm and buttery popcorn. But you can’t always get those fresh flavors, which is where processed foods come in. However, the preservation methods that give processed foods shelf life tend to reduce flavor. So food manufacturers turn to add natural flavors to enhance or maintain the flavor of the food after it’s processed. With that in mind, how do you classify these flavors on labels?

Consumers are getting smarter and savvier when it comes to their food. Regulations for labels constantly change. Both closely examine labels and the requirements for foods to claim certain tags. Consumers also tend to develop more brand loyalty when they feel confident in a food’s ingredient claims. Ensuring you understand the terminology used in specific labels helps classify what your product’s flavor is called on a label. Take a look at our breakdown below to see where your product falls in the category of flavor labeling.

Here’s a list of descriptions of a few key flavor labeling terms used (using Strawberry as an example):

Natural Strawberry Flavor:

Flavors labeled “natural” must only have natural ingredients from the flavor you claim on the label. In this example, the flavors only come from real Strawberries.  This means the essential oil, essence or extractive, protein hydrolysate, or any product of enzymolysis, is derived from a strawberry.

Natural Strawberry (With Other Natural Flavors):

For this label, a flavor contains all natural flavor ingredients, but the flavors are derived from Strawberry as well as other natural flavors. In this instance, Natural Strawberry might be flavored with compounds derived from other berries such as Apple, Raspberry, Blueberry, Pear, etc.

Strawberry Type Flavor:

“Type” appears on labels when you create a flavor from natural ingredients without using the actual label’s flavor. For example, a Strawberry Type flavor is made entirely from natural flavor combinations like Apple, Raspberry, and Pear, but no Strawberry. It’s important to remember this flavor is still derived from all natural ingredients.

Artificial Strawberry Flavor:

You use this particular flavor label when you combine artificial “Strawberry” compounds with other synthetic ingredients.  Artificial flavors are made from non-food sources while natural flavors come from foods or other edible things. The flavor source can contain another natural flavor besides Strawberry, but if the label says “artificial” in it, it includes synthetic “Strawberry” ingredients as well.

Natural and Artificial Strawberry Flavor:

Like it’s name suggests, Natural and Artificial Strawberry flavor contains flavors that naturally come from Strawberry plus other artificial ingredients. The natural flavors come from Strawberry or Strawberry derivatives, or other flavors from a natural source.

Where does your product fit in the list of terminology? Do you have any questions about the flavors in your product? Or want to make any adjustments? Our world-class flavor library and top-rated flavor chemists are ready to help. Call us today to get started.

Sensapure Flavors Announces Spin Off

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Sensapure Flavors Announces Spin Off

December 4, 2017, Salt Lake City, Utah – Sensapure Flavors is now an independent flavor house spin off. This is in conjunction with the sale of Genysis Brand Solutions to Nellson Nutraceuticals, LLC (“Nellson”). It was formerly a division of The Genysis Group.

As part of the agreement with Nellson, Sensapure Flavors still provide powdered flavorings to Genysis Brand Solutions. In a few months, Sensapure Flavors will spin off and relocate to accommodate the needs of the growing business.

“Over the years of sourcing specific flavors from the global providers for our nutraceutical brand partners, we realized that no flavor houses were focused on serving the supplement nutrition industry,” said Jeff Reynolds, founder and CEO of Sensapure Flavors. “The flavoring needs of the nutraceutical industry are complex and ever changing. Being able to launch this company with a leader in the industry like Genysis Brand Solutions is a win/win.”

Our Flavor Chemists

To operate as an independent company and to support the anticipated growth of the business, Sensapure hired certified flavor chemists. This is a first for a Utah based nutrition company, and a team of industry veterans in nutrition. Creating natural and artificial flavors involves thousands of extracts, chemicals and oils. After, you combine those ingredients with active ingredients such as protein, amino acids to make a powder form. This requires a process called spray drying.

So Much Space in Our Spin Off!

The new facility is in the Pioneer Square Business Park at the intersection of I-215 and 2100 South, SLC. It boasts 30K sq ft dedicated to flavor labs, and flavor manufacturing and offices for the Sensapure team. The flavor labs have a flavor creation lab (to create liquid flavors), an applications lab (to test different flavors with functional ingredient bases) and a sensory lab (for taste testing). 

In addition to the Utah headquarters, Sensapure has a location in Southern California with the same suite of flavor laboratories. This R&D lab opens in spring 2018. The Utah campus also houses Dyad Labs, an analytical testing lab also spun off as part of the transition. An open house of the Utah facility is scheduled for Spring of 2018.

About Sensapure Flavors 

Sensapure Flavors is a flavor house serving the nutrition and supplement industry with exciting flavor technology and unmatched applications experience. Initially it was a manufacturing company to understand the ever-changing needs of the nutraceutical industry. Now, Sensapure combines new flavor technologies with deep product application experience. They focused on powdered flavors initially, but now they will serve all flavoring aspects of the dynamic supplement and functional nutrition space.

Nellson Nutraceutical to acquire Genysis

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Nellson Nutraceutical to acquire Genysis

( December 1 , 2017) Anaheim , CA – Nellson Nutraceutical (“Nellson”), a leading North American formulator and manufacturer of branded and private – label nutritional bar and functional powder products, today announced that it has acquire Genysis Brand Solutions Inc. (“Genysis ”). Genysis is a Salt Lake City-based manufacturer of functional powders. Their affiliates Dyad Labs (formerly Genysis Labs) and Sensapure Flavors have been spun out as independent entities and are not included in the transaction.

The best combination

The business combination strengthens Nellson’s powder production capabilities. It expands its product lines and channels, and diversifies its customer mix. “The combination with Genysis, together with the recent opening of our state-of-the-art bar-making facility in Ontario, California further strengthens our position as a strategic partner of choice within the fast growing nutritional bar and functional powders sectors” said Jamie Better, Chief Executive Officer of Nellson. “This acquisition replicates in powders what we achieved in bars three years ago through our acquisition of Multibar, as it combines two respected functional powder manufacturers to create the clear category leader with remarkable capabilities in new product development and best-in-class manufacturing.”

An Exciting Acquire

Genysis operates as a subsidiary of Nellson. Adam Schwinghammer, President of Genysis, who stayed on as a key member of the executive management team, said: “We are excited about merging with Nellson, and capitalizing on the respective strengths of the two companies in order to benefit our current and prospective customers.”

The acquired company has five manufacturing and R&D facilities. This offers greater capabilities to customers in the U.S., Canada, and internationally.

Nellson is a portfolio company of Kohlberg & Company, L.L.C (“Kohlberg”), a leading private equity firm headquartered in New York.

Seth H. Hollander, Partner at Kohlberg, commented: “Since our acquisition of Nellson in 2013, we focused on identifying acquisitions as a means to grow and expand our capabilities, product offering and customer base. Genysis represents a key step toward that end.” Hollander added, “Nellson and Genysis are highly complementary businesses. We plan to capitalize on this unique opportunity to create a larger, more diversified company capable of better serving our combined customer bases.”

Antares Capital provided Nellson with fully committed credit facilities sufficient to close the transaction. Paul, Weiss, Rifkind, Wharton & Garrison LLP acted as legal counsel to Nellson. Houlihan Lokey acted as financial advisor to Genysis.

About Nellson

Nellson, the leading full-service nutritional bar and powder provider in North America, is based in Anaheim, California. They have production locations in California and Quebec. Nellson has over 50 years of diversified expertise since 1962. They specialize in nutrition platforms for bars and powders. They serve the nutrition, performance, weight management, snack/breakfast, and functional market segments. Nellson offers technical capabilities, quality assurance, flexible production, research and development, and sales and marketing support.

Visit www.nellsonllc.com for more information.

About Genysis

Founded is 2003 in SLC, Genysis is a premier producer of powdered nutritional supplements in North America. Genysis offer customers a diverse base of products, and are recognized in the industry for exceptional quality, consistency and reliability of service. In addition, the Company places a strong emphasis on R&D, with tremendous institutional knowledge based on over 10 years of functional powder manufacturing expertise. Genysis operates a Salt Lake City facility comprising over 300,000 square feet of production and warehouse space.

For more information visit www.Genysisgroup.com

 About Kohlberg & Company

Kohlberg & Company is a leading private equity firm that specializes in middle market investing. Since its inception in 1987, Kohlberg organized eight private equity funds, through which it raised over $ 7 .5 billion of committed equity capital. Over its 30-year history, Kohlberg completed 72 platform investments and well over 162 add-on acquisitions, with an aggregate transaction value of over $15 billion.

For more information, visit www.kohlberg.com

Nellson LLC
5115 E. La Palma Ave.
Anaheim, CA 92807
Phone: 714.765.7000
Website: NellsonLLC.com

1945 S. Fremont Drive
Salt Lake City, UT 84104

© 2018 Sensapure Flavors, All Rights Reserved

(801) 456-4284
[email protected]